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Sunday, November 29, 2020

Molecular diagnosis.

Molecular diagnosis  

It is well known that an early diagnosis is very important for effective treatment of the disease


Methods of Molecular Diagnosis

1) Tradition method include conventional report in which early detection is not possible. This involve serum and urine analysis.

2) Molecular method include modern methods in which early detection is possible. They involve recombinant DNA technology (RDT), polymerase chain reaction etc. 

Some of the popular test to diagnose a particular disease 

 Polymerase chain reaction -: This method was developed in 1985 by Kary Mullis   
                                       
Polymerase chain reaction


Principle :- 
PCR is a process of amplification of the nucleic acid. In         this method nucleic acid(DNA) of bacteria is taken amplification. It is important to note that the low concentration of bacteria or virus can be easily detected by amplification of their nucleic acid.

Applications :-
PCR is now routinely used to detect HIV in suspected AIDS patient .
It is being used to detect mutation in genes of suspected cancer patients. PCR is used to detect genetic diseases in foetuses before their birth. 

Polymerase chain reaction








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